{"id":38073,"date":"2026-03-05T09:00:00","date_gmt":"2026-03-05T08:00:00","guid":{"rendered":"https:\/\/www.etap-lab.com\/?post_type=ressource&#038;p=38073"},"modified":"2026-05-13T12:12:08","modified_gmt":"2026-05-13T10:12:08","slug":"publication-animal-origin-free-method-for-generating-blood-vessel-organoids","status":"publish","type":"ressource","link":"https:\/\/www.etap-lab.com\/en\/ressource\/publication-animal-origin-free-method-for-generating-blood-vessel-organoids\/","title":{"rendered":"Publication: &#8220;Animal-origin-free method for generating blood vessel organoids&#8221;"},"content":{"rendered":"\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p>Publication on <strong>Animal-origin-free method for generating blood vessel organoids<\/strong>. Hoffmann, A., Schorn, D., Th\u00f6nig, J.\u00a0<em>et al.<\/em>. Published in <strong>Sci Rep 16, 12096 (2026)<\/strong>.<\/p>\n\n\n\n<p>Read the full article: <a href=\"https:\/\/doi.org\/10.1038\/s41598-026-42977-z\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.1038\/s41598-026-42977-z<\/a><\/p>\n\n\n\n<p><strong>Authors<\/strong>: Alexander Hoffmann1,2, David Schorn1, Jakob Th\u00f6nig1, Yu-Hsiang Teng1, Jean-Fran\u00e7ois Bisson3 &amp; Teodor E. Yordanov1<\/p>\n\n\n\n<p><strong>Affiliated<\/strong>:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>1 Angios FlexCo, Exlgasse 24, 6020 Innsbruck, Austria. <\/li>\n\n\n\n<li>2 Department of Internal Medicine II, Medical University Innsbruck, Innsbruck, Austria. <\/li>\n\n\n\n<li>3 Department of Dermatology, ETAP-Lab, Vandoeuvre-l\u00e8s-Nancy, France. <\/li>\n<\/ul>\n\n\n\n<p><\/p>\n\n\n\n<p><strong>Abstract:<\/strong><\/p>\n\n\n\n<p>Blood vessel organoids (BVOs) represent a promising tool for modeling vascular diseases, drug screening, and regenerative therapies. However, current protocols for BVO generation are complex, labor-intensive, and reliant on animal-derived extracellular matrices (ECM) such as Matrigel, limiting reproducibility, scalability, and clinical applicability. We developed a simplified, animal-origin-free protocol for BVO generation that addresses current limitations and enables high-throughput automated workflows. The method employs ultra-low attachment 96-well U-bottom plates for standardized aggregation and differentiation of human induced pluripotent stem cells (hiPSCs) in a human derived collagen-based extracellular matrix. Unlike conventional protocols where aggregates are embedded in a two-layer ECM, our approach utilizes a single-layer, which we termed \u201csitting drop\u201d. This innovative approach requires considerably fewer materials and handling steps and is compatible with high-throughput automated machines. BVO generation utilizing the here described optimized protocol resulted in the formation of BVOs with reproducible morphology and cellular composition. Flow cytometry confirmed the presence of CD31\u207a endothelial cells and PDGFR\u03b2\u207a pericytes in BVOs, generated in sitting drops in ultra-low adhesive U-bottom shaped 96 well plates, with cell population percentages comparable to those observed in traditional two-layer BVO cultures. In vivo transplantation of mature BVOs in a mouse full-thickness skin wound model demonstrated integration of BVO derived cells into host vessels, highlighting their potential in cell-based therapies. Our study presents a robust and animal-origin-free method for BVO generation based on single-layer \u201csitting drop\u201d cultures. This protocol maintains cellular integrity while enhancing reproducibility and automation-readiness, paving the way for high-throughput screening and clinical translation of vascular organoid technology.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><strong><em>Keywords: <\/em>Blood vessel organoids (BVO), Extracellular matrix (ECM), Collagen, High-throughput, Organoids, Bioengineering<\/strong><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Discover Publication on Functional discrimination of CSF from Alzheimer\u2019s patients in a brain on chip platform: Publication : Functional discrimination of CSF from Alzheimer\u2019s patients\u2026<\/p>\n","protected":false},"featured_media":37803,"template":"","meta":{"_acf_changed":false},"categorie-de-ressource":[235],"marque-de-ressource":[241],"class_list":["post-38073","ressource","type-ressource","status-publish","has-post-thumbnail","hentry","categorie-de-ressource-publications","marque-de-ressource-skinaxis"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Publication: &quot;Animal-origin-free method for generating blood vessel organoids&quot; - ETAP-LAB<\/title>\n<meta name=\"description\" content=\"Publication on Animal-origin-free method for generating blood vessel organoids. 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