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Preclinical in vivo Dermatology Research Services

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With over two decades of expertise, ETAP-Lab stands as a recognized leader in preclinical dermatology. Leveraging our gold standard models and tailor-made solutions, we address your challenges in molecule development, spanning across areas such as psoriasis, atopic dermatitis, wound healing & skin grafting. Additionnaly we propose UV-induced models, pain models and dermal toxicity.

In the studies we design, in addition to macroscopic scoring, the benefits of treatment are confirmed through translational models empowered by behavioral approaches.

Contact our experts to find out about our standard to customized models & services.

Inflammation & Immuno-Dermatology

ETAP-Lab has strong expertise in Skin Inflammation and Immuno-Dermatology, and provides unique rodent model in mice. Learn more by walking through our experimental specifications, image gallery and scientific publications. We provide other gold standard models of Psoriasis, Atopic Dermatitis and Eczema, as well as Actinic Keratosis and Hyperkeratosis.

You are looking for more information? Feel free to contact our experts!

Experimental Specifications

Chronic Skin Inflammation

Animals: female Hairless mice

Induction of chronic skin inflammation: daily topical application of TPA for 1 week

Treatments: start one week after the start of the induction of chronic skin inflammation

Tested products: solutions (oral or topical route), dressings, creams, ointments, film-forming (application or spraying) and hydrogels

Followed parameters: macroscopic scoring of skin inflammation, histopathological analysis, microscopic scoring of skin inflammation and dosages of cytokines (IL-1β, IL- 6, TNF-α...)

Reference substances possible: Tridesonit, compounds and dressings depending on the nature of products to test and on the treatment route

Atopic dermatitis

ETAP-Lab has strong expertise in atopic dermatis and provides unique model in mice allowing to demonstrate the efficacy of products and ingredients on this skin disorder.

To complete the classic inflammatory biomarker assays (cytokines, immunoglobulins, infiltration of immune cells, histamine) and pathological methods, ETAP-Lab has developed specific scoring scales to allow a detailed analysis of the results, and the study of rodent behaviour which allows to evaluate the animals’ pain and well-being and to differentiate the activity of products tested.

Contact our experts to find out about our testing and consultancy services

Experimental Specifications

➡️ Animals: mice

➡️ Induction: topical application of DNCB

➡️ Treatments: systemic or topical route

➡️ Followed parameters: Body weight, MBWC, score of the use of enrichment, Macroscopic scorage of atopic dermatitis severity (ADSI​), Measurements of TransEpidermal Water Loss (TEWL)​, Dosages of cytokines (IL-1β, IL-6, IL-18, IL-33, TNF-α…), IgE and Histamine​; Behavioral evaluation (scratching, licking, rearing, distance travelled)​; Histology, immunohistochemistry and immunoblotting analyses​…

➡️ Reference substances: Corticosteroids, corticoids, emollients, immunomodulators, immunosuppressors.

Publications on the topic

Psoriasis

ETAP-Lab has strong expertise in Skin Inflammation, especially in  Psoriasis, and provides a rodent model in mice enriched with an unique behavioral scoring of scratching and licking. Learn more by walking through our experimental specifications, image gallery and scientific publications.

You are looking for more information? Feel free to contact our psoriasis experts.

Experimental Specifications

Animals: female Balb/c mice.

Induction of psoriasis: topical application of Imiquimod daily for one week.

Treatments: start five days after the start of induction of psoriasis.

Tested products: solutions (oral or dermal route), creams, ointments, dressings, hydrogels (+ neutral covering dressing).

Followed parameters: body weight, macroscopic scoring of psoriasis (several parameters), cytokine assays (IL-1β, IL-6, IL-17, IL-22, TNF-α), histopathological analysis, microscopic scoring of psoriasis.

Additional parameter: behavioral evaluation (scratching and licking of the back).

Reference substances possible: clobetasol, calcipotriol, compounds and dressings depending on the nature of products to test and on the treatment route.

Skin grafting

ETAP-Lab is a European leader in experimental skin grafting, with a laboratory dedicated to preclinical dermatology. For the past 5 years, we have been working with direct customers across Europe to develop innovative grafting models for regenerative therapy.

Our preclinical research aims to support our customers and partners in regenerative medecine aiming at treating ulcerated wounds, major burns and genetic skin diseases.

You are looking for more information? Feel free to contact our experts.

Experimental Specifications

We already offer 9 original models of complete excisional wounds, with a variety of shapes and devices, enabling the use of skin substitutes.

➡️ Species: immunocompetent and immunodeficient (Nude) mice and rats
➡️ Induced by surgery.
➡️ Advanced grafts: Autografts, Isografts, Allografts & Heterografts (xenografts, e.g. from human to mouse)
➡️ Shapes and devices: circular with silicone ring, Healobs Sil ® device (4 shapes), with flap, with devitalized skin… 
➡️ 0% graft rejection rate: thanks to our know-how in respecting circadian rhythms, ethics and chronobiology of rodents.
➡️ Followed parameters: Graft survival, Surface area of the grafted site, TEWL, Histological analysis, Immunohistochemical analysis (CD31, Ki67, Collagen…)… 

Wound healing

ETAP-Lab has strong expertise in Wound healing and provides unique rodent models in normal and diabetic mice or rats. Learn more by walking through our experimental specifications and scientific publications.

In addition, we also propose original models of Skin abrasion, Blisters / Burns, and Fibrinous wound.

You are looking for more information? Feel free to contact our wound healing experts.

Experimental Specifications

Excisional skin wound

One of our gold standard used for more than 20 years.

➡️ Species: healthy and diabetic mice and rats
➡️ Surgery: skin biopsy of 6 to 26 mm diameter on the back under anesthesia
➡️ Treatment start immediately after the skin biopsy
➡️ Tested products: solutions (Dermal or oral routes), creams, foams, ointments, hydrogels (+ neutral covering dressing), dressings
➡️ Large choice of parameters to follow: blood glucose/insuline levels, tolerance and efficacy scoring, surface area of the wound, determination of the day of complete healing, histopathological analysis, microscopic scorage of wound healing, immunohistochemistry: Ki-67, CD31, Collagen I, III, IV…
➡️ Reference substances possible: hyaluroinic acid (Ialuset®), sulfadiazine Ag, compounds and dressings depending on the nature of products to test and the treatment route considered.

Ischemic ulceration

ETAP-Lab has strong expertise in wound healing, especially in Ischemic ulceration, and provides unique rodent models in normal and diabetic mice and rats. Learn more by walking through our experimental specifications, image gallery and scientific publications.

You are looking for more information? Feel free to contact our experts.

 

➡️ Animals: normal or diabetic mice and rats
➡️ Induction of ischemic ulceration: mechanically on the back of animals in 2 steps, with or not resection of the ischemic region
➡️ Treatments: start immediately after induction of ischemic ulceration
➡️ Tested products: solutions (oral or topical route), dressings, creams, ointments and hydrogels (+ neutral covering dressing)
➡️ Followed parameters: macroscopic scoring of ischemic ulceration (several parameters), surface area of the ischemic region, determination of the day of complete healing and possibly histopathology depending on the study
➡️ Reference substances possible: compounds and dressings depending on the nature of products to test and on the treatment route

Leading CRO for preclinical dermatology research

ETAP-Lab has been a recognized leader in preclinical dermatology for over 20 years, working for long-term customers among pharmaceutical, biotech and medical device companies. The company has built up one of the broadest portfolio of in vivo models with 30 different models available, mainly in the fields of skin inflammation, immunodermatology and wound healing, and for the past 5 years in the field of skin grafting.

Expertise in building original translational in vivo models

The company offers high-quality preclinical services with high translational value, supported by study designs tailored to meet specific customer needs. We design our studies to provide optimal translational situations: experimental conditions that respect chronobiology, chronopharmacology and animal welfare. Treatment benefits are confirmed by translational models backed up by behavioral approaches.

Dedicated expert team at the forefront of dermatological science

Innovation drives us to continually update our knowledge and techniques on key future healthcare issues. Active member of dermatological associations such as European Tissue Repair Society (ETRS), Société Française de Toxicologie (SFT), European Wound Management Association (EWMA) and European Society for Dermatological Research (ESDR).