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in vitro & in vivo Neurology

Since its inception, ETAP-Lab – preclinical CRO – has demonstrated solid expertise in preclinical neurology, initially focusing on in vivo models with historical behavioral expertise, we offer efficacy models with high translational approach taking into account most risk factors. We have extended our know-how to in vitro approaches, with a broad portfolio of cellular models of Neurodegenerative diseases and proteinopathies such as Alzheimer’s and Parkinson’s disease. Our services include advanced high-content screening, bioassays & histology platform, fully-equipped with wide variety of analyzes & biomarkers. We offer a comprehensive range of solutions, from standard to customized to meet your specific needs, in the fields of Neuroscience & CNS – in stroke, neurodegenerative diseases and psychopharmacology.

Our models by area of expertise

in vitro models

Protein aggregation (cell free) | Induction with AβO, TauO or αSO
Excitotoxicity* | Glutamate injuries
Oxidative stress* | H₂O₂-induced
Neurogenesis*
Synaptogenesis*
Neuroinflammation (Human iPSC Astrocyte) | Induction with AβO or Cytokine
Neurodegeneration*| Induction with AβO, TauO or αSO, or 6-OHDA

READOUTS
Diagnostic of amyloid structure | Cell viability | Neurite network | Synaptic density | Astrogliosis| Inflammation signaling pathway | Proliferation

 

in vivo models

Amyloidopathy model | AβO-induced on mice
Amyloidopathy & Aging Model | AβO-induced on mice
Amnesia Model | Pharmacologically induced on mice
Parkinson-Like Model | 6-OHDA-Induced on rats
Parkinson-Like Model | Lactacystin-Induced on rats
Catalepsy model | Pharmacologically induced on rats
Dyskinesia model | L-dopa-induced on rats

 

READOUTS
Memory impairments | Sensorimotor deficits | Synaptic loss | Astrogliosis | Microglia activation

 

*Cortical Primary Neurons. Proteinopathies models in blue.
AβO for Amyloid Beta Oligomers. TauO for Tau Oligomers. αSO for Alpha Synuclein Oligomers.

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in vitro Models

Rotational Thromboelastography (ROTEM) | Human whole blood

Clot lysis assay |Human blood

Excitotoxicity-induced cellular stress | Glutamate

MAIN READOUTS
Clot formation and lysis parameters | Lysis time | Cell viability | Neurite network | Synaptic density

 

in vivo Models on mice & rats

Distal Ischemic Stroke models
Thrombin model | Fibrin-rich clot thrombosis

FeCl3 or AlCl3 models | Platelet-rich clot thrombosis

Hemorrhagic transformation following ischemic stroke model (with a comorbidity)

 

Proximal Ischemic Stroke models
Thrombo-embolic stroke model | Multiclot version

Transient middle cerebral artery occlusion model (tMCAo)

 

Hemorrhagic Stroke models

Collagenase model | Vascular rupture

In situ infusion of heterologous blood or autologous blood | No vascular rupture

Intracerebral aneurysms

 

MAIN READOUTS
Lesion volume | Oedema volume | Hemorrhage occurrence and volume | Molecular magnetic resonance imaging | Neuroscore | Motor coordination | Spontaneous and exploratory activity | Hemoglobin concentration in the tissue (Drabkin assay) | Acute superficial tissue perfusion (CBF index) | MCA recanalization | Brain tissue perfusion | Blood brain barrier integrity | Neurovascular coupling following whisker stimulation | Sensorimotor tasks |(Immuno)histology or bioassays on brain tissue.
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in vivo models

 

MOOD DISTURBANCES MODELS

 

Anxiety models | Induction options: Elevated plus Maze test, Defensive burying test, Light/Dark box test,

Marble burying test or Chronic mild stress induction

Depression models | Induction with Chronic mild stress induction

Learning models | Induction with Morris Water Maze test or Novel Object Recognition.

Locomotor activity | Induction with Open-field test

 

MOTOR DISTURBANCES MODELS

 

Sensorimotor assessment | Induction with Rotarod

 

MAIN READOUTS

Physiological monitoring & video tracking data | Sucrose preference Test | Behavioural scoring | Memoryand Motor performances assessement | Dosage in CSF, blood, brain and organs

Neuro-PharmacoKinetic (Neuro-PK) models | Delivery of a pharmacological compound. All administration routes (PO, IV, IP, IN, IT, SC).

 

MAIN READOUTS

Dosage in CSF, blood, brain and organs

Our platforms

Microsurgery facilities
Immunohistochemistry Plateform
Biological Assays

Behavioural tests

Stereotaxic & micro-surgery

in vivo Imaging

Histology & Immunohistochemistry

Biological assays

In vitro pharmacology

Our R&D Projects

Bio-Diamond

The BIO-DIAMOND project stands as a pioneering pilot initiative showcasing the potential of organ-on-chip technology to expedite the market entry of drug candidates in the “discovery” and “pre-clinical” phases, specifically focusing on Alzheimer’s, Parkinson’s, and Amyotrophic Lateral Sclerosis (ALS).

The project, in collaboration with NETRI, has emerged as a winner of the “Innovations in biotherapies and bioproduction” call for projects funded by the French government as part of France 2030.

BrioFlam

This collaborative project with Plant Advanced Technologies (PAT) and the IMoPA-CNRS laboratory at the University of Lorraine aims to discover new plant-derived anti-inflammatory compounds, with a special emphasis on bryophyte group plants.

Selected by the Grand Est Region and the State, this collaborative research initiative is backed by a total budget of 1.2 million euros.

STROKTURA

STROKTURA, Stroke for the Future, aims to find innovative solutions in brain imaging applied to preclinical models of stroke, with the purpose of testing future therapies for ischemic strokes in humans. This project, led by our team of experts in collaboration with BB@C, with scientific and technical support from PhIND, ESR3P, and CYCERON, is financially supported by FEDER and the Normandy Region.